Phosphorylated H2AX (γ-H2AX) is a sensitive marker for DNA double-strand breaks (DSBs), but the variability of H2AX expression in different cell and tissue types makes it difficult to interpret the meaning of the γ-H2AX level.

H2AX and Western Blot (Lab E214) Gamma H2AX foci Group 4 starts experiment, other groups learn to score chromosomal aberration/micronuclei/γ-. H2AX

The gamma-H2AX Pharmacodynamic Assay Kit is for the study of double strand DNA breaks through the detection of gamma-H2AX in peripheral blood mononuclear cells, cultured cells, and tissue biopsies. Contract Services are also available. Phosphorylated on Ser-140 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) also known as H2AXS139p.

2010-02-04 · In short, γH2AX foci act as useful surrogate markers of DNA damage induced by these genotoxic agents. 111, 115, 121. In the context of cancer therapy, quantitation of γH2AX is a particularly Histone H2AX phosphorylation on a serine four residues from the carboxyl terminus (producing gammaH2AX) is a sensitive marker for DNA double-strand breaks (DSBs). DSBs may lead to cancer but, paradoxically, are also used to kill cancer cells. Using gammaH2AX detection to determine the extent of DSB induction may help to detect precancerous cells, Gamma H2AX (γH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage.

2007-02-06

Phospho-H2AX or γ-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues.

How phosphorylated histone H2AX, known as γ-H2AX, functions in the cellular response to DNA double-strand breaks is the subject of intensive investigation.

Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). Phospho-H2AX or γ-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. The results suggest gamma-H2AX is a useful adjunct in diagnosis of metastatic RCC when RCC-Ma is negative and in higher grade RCC, which are often a diagnostic challenge. A nuclear pattern of staining of gamma-H2AX has a comparable sensitivity with RCC-Ma, and the interpretation is easier and more reliable. Therefore, the analysis of H2AX phosphorylated (γH2AX) may be possibly used as biomarker of genotoxicity and genomic instability with a number of applications in human epidemiology.

Keywords: Gamma-H2AX, High-content screening, Image processing, Hough transform, dosimetry 1. INTRODUCTION The study of damage to cellular DNA is essential for the understanding of cell apoptosis and mutation that may lead to serious disease including cancer. Changes in the genome caused by a single double strand break (DSB) may be enough Anti-Gamma H2AX (phospho-Ser139) antibody, Rabbit monoclonal. 1 Product Result | Match Criteria: Product Name, Property The detection of gamma-H2AX can potentially serve as a biomarker for transformation of normal tissue to premalignant and consequently to malignant tissues. gamma-H2AX has already been investigated 2019-08-22 2012-07-23 Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb in the presence of control peptide (left) or Phospho-Histone H2A.X (Ser139) Blocking Peptide #1260 (right).
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View specifications, prices, citations, reviews, and more. gamma-H2AX (gH2AX) is the phosphorylated form of histone H2AX and can function as a sensitive marker for double-strand breaks (DSBs), which signifies genomic instability and can potentially contribute to cancer initiation and progression.

PMID 16310392 : Tyrosine dephosphorylation of H2AX modulates apoptosis and survival decisions. HT gamma -H2AX Pharmacodynamic Assay 4418-096-K 2 Citations Submit a Review Datasheet / COA / SDS For the quantitation of gamma-H2AX levels in peripheral blood mononuclear cells, tissue culture cells, and tissue biopsies Kinner A, Wu W, Staudt C, Iliakis G (2008) Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic Acids Res 36:5678–5694 PubMed CrossRef Google Scholar Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 is a highly specific mouse monoclonal antibody, that targets Histone H2A.X & has been tested in western blotting, ICC, ChIP & Immunofluorescence. Abstract.
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Bethyl Laboratories Anti-gamma-H2AX Recombinant Monoclonal (BLR053F), Catalog # A700-053. Tested in Western Blot (WB), Immunofluorescence (IF),

Total lysates (15 µg protein) from untreated HeLa (Lane 1) and UV treated HeLa (Lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL Purified anti-H2A.X Phospho (Ser139) antibody, clone 2F3 (upper) or 1:2000 diluted anti-GAPDH antibody, clone 1D4 (lower). The DNA double-strand break (DSB) is the primary lethal lesion after therapeutic radiation. Thus, the development of assays to detect and to quantitate these lesions could have broad preclinical and clinical impact. Phosphorylation of histone H2AX to form γ-H2AX is a known marker for irradiation-induced DNA DSBs.

Anti-phospho-H2A.X (Ser139) Antibody Upstate®, from rabbit; Synonym: H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac; find Sigma-Aldrich-07-164 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.

H2AX (H2A.X Variant Histone) is a Protein Coding gene. Diseases associated with H2AX include Nijmegen Breakage Syndrome and Ataxia-Telangiectasia. Among its related pathways are Signaling by GPCR and Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3.

in the structure and spatial organization of chromatin, gamma-H2A. Däremot detekterades fosforyleringar av ATM och y- H2AX i både 6 Gy uppvisade inte ACOT7 Si-transfekterade celler gamma-H2AX-foci i kärnan (figur 4e). Latexin sensibiliserar leukemogena celler till gamma-bestrålningsinducerad PE-konjugerade antikroppen p-histon H2AX (S139) (Cell Signaling, Danvers, MA I själva verket, strax efter IR, berikades γ- H2AX normalt i foci i Mcl-1 eller IEX-1-bristfälliga celler. Detta överensstämmer med det faktum att IEX-1-expression γ -H2AX: Gamma, H2A histone family, member X. 3′UTR: 3′-Untranslated region. 5-Fu: 5-Fluorouracil. ABCB9: ATP-binding cassette, subfamily B (MDR/TAP) alpha, IFN-gamma-inducible protein 10 (IP-10), IL-lb, his Gamma-aminobutyric acid (GABA) H2AX Foci in Human Lymphocytes from Hypersensitive and. Microwaves from UMTS/GSM mobile phones induce long-lasting inhibition of 53BP1/gamma-H2AX DNA repair foci in human lymphocytes.